Yourgene Health (a part of the Novacyt group of corporations), a number one worldwide molecular diagnostics group, and PacBio, a number one developer of high-quality, extremely correct sequencing options, at the moment introduced a collaboration to optimize dimension choice for long-read sequencing workflows. PacBio has certified Yourgene’s LightBench^® instrument for dimension number of lengthy DNA fragments, enabling excessive yields for HiFi sequencing information.

Yourgene’s LightBench is an automatic electrophoresis instrument, powered by Ranger^® Technology. This platform makes use of a patent-protected, machine-vision algorithm to interpret and management gel electrophoresis in actual time. It delivers correct dimension choice for human genomic DNA and consists of the power to carry out fragment size evaluation for added high quality management.

PacBio HiFi sequencing is a single-molecule real-time (SMRT^®) sequencing know-how. It produces lengthy (1,000 to twenty,000+ bases), correct reads with uniform genomic protection and native methylation detection to be used in purposes reminiscent of entire genome reconstruction, haplotype phasing, detection of enormous and complicated variants, and epigenetics.

A brand new Technical Note was launched at the moment for PacBio clients titled “Size selection of PacBio SMRTbell^® libraries with the LightBench^® instrument from Yourgene Health.” The Yourgene LightBench instrument is suitable with the Revio system. The automated LightBench workflow optimizes stroll away time, providing a scalable dimension choice resolution for the preparation of PacBio SMRTbell sequencing libraries. Results from the preliminary research assessing two fragment lengths in 84 human genomic DNA samples from frozen blood for library preparation confirmed constant yields for 8 kb or 10 kb dimension and a zero % failure charge.

PacBio HiFi sequencing know-how is a recreation changer for entire genome purposes. High-resolution dimension choice is essential to maximizing information yields from long-read sequencing and Yourgene is proud to supply researchers a exact and scalable resolution with the LightBench.”

Matthew Nesbitt, Co-Founder of Ranger Technology, Yourgene Health